12th Annual IGSS Conference • October 28-29, 2021

Integrating Genetics and the Social Sciences 2021

DNA methylation analysis using the Illumina MethylationEPIC arrays

Allison Kupsco, Associate Research Scientist
Haotian ("Howie") Wu, Postdoctoral Research Scientist

Laboratory of Precision and Environmental Health, Columbia University Mailman School of Public Health

This intensive half-day workshop will provide participants with a solid foundation in DNA methylation analyses for human population studies. Using hands-on R lab sessions, this workshop will integrate important conceptual and study design considerations where participants will work with real methylation data generated by the Illumina Infinium MethylationEPIC (850k) BeadChip platform. Participants will learn data handling, quality control, and basic analysis of DNA methylation data for epigenome-wide association studies (EWAS), including epigenetic clocks and regional analyses. Participants should leave this course prepared to undertake basic EWAS analyses. A working knowledge of R programming and basic knowledge of biostatistics are recommended for this course.

Workshop Slides: Part 1 (Wu)   Part 2 (Kupsco)

Workshop Materials (Zip file, 300 Mbs)

Download the IGSS_Epigenetics_Course_2021.zip file to your computer's desktop. Extract the contents to a folder on your hard drive. Run the "IGSS_2021_install_packages FINAL.r" script. It can take up to a couple of hours to install all the necessary R packages, so please allow plenty of time before the workshop. We also recommend that you have ~4G of RAM available for the analyses.